Verbascoside production in long-term Buddleja cordata Kunth cell suspension cultures.

Previously, our group reported the institution of a white callus cell line of Buddleja cordata Kunth that may be a excessive producer of the secondary metabolite, verbascoside (VB, additionally named acteoside), below suspension tradition situations.

Here, we current experimental proof of the sustained capability of that mobile line to develop and produce excessive quantities of VB for five years of steady tradition. Cellular line profiles had been decided on the early (at first) and late phases (on the finish of 5 years of steady subculturing) by analyzing related parameters of tradition development, i.e., particular development charge [µ], doubling time [dt], and development index [GI], in addition to VB manufacturing.

Late-stage cultures exhibited a 61% sooner development charge than early-stage subcultures, and 25 and three% decrease doubling time and development index. The extents of development phases had been discovered to be totally different.

EIF4G1 Antibody

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EIF4G1 Antibody

ABD7614 100 ug
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EIF4G1 siRNA

20-abx915213
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20-abx915214
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eIF4G1 Antibody

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EUR 540

eIF4G1 Antibody

AF7850 200ul
EUR 540

EIF4G1 Antibody

1-CSB-PA780667
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

eIF4G1 Antibody

DF7614 200ul
EUR 420

eIF4G1 Antibody

AF2641 100ul
EUR 420

EIF4G1 Antibody

1-CSB-PA243767
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

EIF4G1 Antibody

1-CSB-PA007568GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

EIF4G1 Antibody

1-CSB-PA007999
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

eIF4G1 Antibody

DF7764 200ul
EUR 420

anti-EIF4G1

YF-PA23636 50 ul
EUR 400.8
Description: Mouse polyclonal to EIF4G1

anti-EIF4G1

YF-PA11524 50 ug
EUR 435.6
Description: Mouse polyclonal to EIF4G1

EIF4G1 Antibody

RQ5515 100 ug
EUR 419

Anti-EIF4G1 Antibody

A01351-2 100ug/vial
EUR 400.8

EIF4G1 Rabbit pAb

A0881-100ul 100 ul
EUR 460.8

EIF4G1 Rabbit pAb

A0881-200ul 200 ul Ask for price

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A0881-20ul 20 ul Ask for price

EIF4G1 Rabbit pAb

A0881-50ul 50 ul
EUR 318

EIF4G1 Blocking Peptide

33R-6989 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EIF4G1 antibody, catalog no. 70R-4875

EIF4G1 Blocking Peptide

20-abx161839
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

EIF4G1 (pS1232) Antibody

20-abx000330
  • EUR 594.00
  • EUR 844.80
  • EUR 427.20
  • 100 ul
  • 200 ul
  • 50 ul

EIF4G1 (pS1148) Antibody

20-abx121476
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

EIF4G1 Rabbit pAb

A7552-100ul 100 ul
EUR 369.6

EIF4G1 Rabbit pAb

A7552-200ul 200 ul
EUR 550.8

EIF4G1 Rabbit pAb

A7552-20ul 20 ul
EUR 219.6

EIF4G1 Rabbit pAb

A7552-50ul 50 ul
EUR 267.6

EIF4G1 Blocking Peptide

DF7614-BP 1mg
EUR 234

EIF4G1 Conjugated Antibody

C37544 100ul
EUR 476.4

Anti-EIF4G1 antibody

PAab02724 100 ug
EUR 426

anti-EIF4G1 (3A10)

LF-MA10096 100 ug
EUR 435.6
Description: Mouse monoclonal to EIF4G1

anti- EIF4G1 antibody

FNab02724 100µg
EUR 606.3
Description: Antibody raised against EIF4G1

Anti-EIF4G1 antibody

STJ116294 100 µl
EUR 332.4
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 antibody

STJ29690 100 µl
EUR 332.4
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 antibody

STJ23520 100 µl
EUR 471.6
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 (2A9)

YF-MA20178 100 ug
EUR 435.6
Description: Mouse monoclonal to EIF4G1

EIF4G1 (pS1148) Blocking Peptide

20-abx162409
  • EUR 376.80
  • EUR 610.80
  • 1 mg
  • 5 mg

Human EIF4G1 shRNA Plasmid

20-abx951370
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Phospho-eIF4G1 (Ser1147) Antibody

AF7349 200ul
EUR 540

Phospho-eIF4G1 (Ser1185) Antibody

AF7350 200ul
EUR 540

EIF4G1 (Ab-1232) Antibody

CSB-PA061737-
EUR 402
Description: A polyclonal antibody against EIF4G1 (Ab-1232). Recognizes EIF4G1 (Ab-1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

EIF4G1 (Ab-1232) Antibody

CSB-PA061737-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against EIF4G1 (Ab-1232). Recognizes EIF4G1 (Ab-1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Phospho-EIF4G1 (Ser1232) Antibody

CSB-PA987086-
EUR 402
Description: A polyclonal antibody against Phospho-EIF4G1 (Ser1232). Recognizes Phospho-EIF4G1 (Ser1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Phospho-EIF4G1 (Ser1232) Antibody

CSB-PA987086-100ul 100ul
EUR 434.4
Description: A polyclonal antibody against Phospho-EIF4G1 (Ser1232). Recognizes Phospho-EIF4G1 (Ser1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

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20-abx980560
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  • 150 µg
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Phospho-eIF4G1 (Ser1147) Antibody

AF2341 200ul
EUR 420

Phospho-EIF4G1 (S1148) Antibody

1-CSB-PA007998
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-EIF4G1 (S1148). Recognizes Phospho-EIF4G1 (S1148) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

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EF009355 96 Tests
EUR 826.8

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ELI-42147m 96 Tests
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ELI-13400h 96 Tests
EUR 988.8

Phospho-EIF4G1-S1232 Rabbit pAb

AP0279-100ul 100 ul
EUR 460.8

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EUR 664.8

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EUR 318

Phospho-EIF4G1-S1108 Rabbit pAb

AP0796-100ul 100 ul
EUR 460.8

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AP0796-200ul 200 ul
EUR 664.8

Phospho-EIF4G1-S1108 Rabbit pAb

AP0796-20ul 20 ul
EUR 219.6

Phospho-EIF4G1-S1108 Rabbit pAb

AP0796-50ul 50 ul
EUR 318

EIF4G1 ORF Vector (Human) (pORF)

ORF018839 1.0 ug DNA
EUR 790.8

Eif4g1 ORF Vector (Mouse) (pORF)

ORF043791 1.0 ug DNA
EUR 1886.4

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ORF043792 1.0 ug DNA
EUR 1886.4

Anti-Phospho-EIF4G1-(S1108) antibody

STJ117894 100 µl
EUR 471.6
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-Phospho-EIF4G1-(S1232) antibody

STJ22122 100 µl
EUR 471.6
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

EIF4G1 sgRNA CRISPR Lentivector set (Human)

K0670901 3 x 1.0 ug
EUR 406.8

Eif4g1 sgRNA CRISPR Lentivector set (Mouse)

K4684101 3 x 1.0 ug
EUR 406.8

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 1)

K0670902 1.0 ug DNA
EUR 184.8

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 2)

K0670903 1.0 ug DNA
EUR 184.8

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 3)

K0670904 1.0 ug DNA
EUR 184.8

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4684102 1.0 ug DNA
EUR 184.8

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4684103 1.0 ug DNA
EUR 184.8

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4684104 1.0 ug DNA
EUR 184.8

EIF4G1 Protein Vector (Human) (pPB-C-His)

PV075353 500 ng
EUR 2995.2

EIF4G1 Protein Vector (Human) (pPB-N-His)

PV075354 500 ng
EUR 2995.2

EIF4G1 Protein Vector (Human) (pPM-C-HA)

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EUR 2995.2

EIF4G1 Protein Vector (Human) (pPM-C-His)

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EUR 2995.2

EIF4G1 Protein Vector (Mouse) (pPB-C-His)

PV175162 500 ng
EUR 3228

EIF4G1 Protein Vector (Mouse) (pPB-N-His)

PV175163 500 ng
EUR 3228

EIF4G1 Protein Vector (Mouse) (pPM-C-HA)

PV175164 500 ng
EUR 3228

EIF4G1 Protein Vector (Mouse) (pPM-C-His)

PV175165 500 ng
EUR 3228

EIF4G1 Protein Vector (Mouse) (pPB-C-His)

PV175166 500 ng
EUR 3214.8

EIF4G1 Protein Vector (Mouse) (pPB-N-His)

PV175167 500 ng
EUR 3214.8

EIF4G1 Protein Vector (Mouse) (pPM-C-HA)

PV175168 500 ng
EUR 3214.8

EIF4G1 Protein Vector (Mouse) (pPM-C-His)

PV175169 500 ng
EUR 3214.8

EIF4G1 3'UTR Luciferase Stable Cell Line

TU006792 1.0 ml
EUR 2799.6

Eif4g1 3'UTR GFP Stable Cell Line

TU253905 1.0 ml Ask for price

Eif4g1 3'UTR Luciferase Stable Cell Line

TU105738 1.0 ml Ask for price

EIF4G1 3'UTR GFP Stable Cell Line

TU056792 1.0 ml
EUR 2799.6

Eif4g1 3'UTR Luciferase Stable Cell Line

TU203905 1.0 ml Ask for price

Eif4g1 3'UTR GFP Stable Cell Line

TU155738 1.0 ml Ask for price

Recombinant Human EIF4G1 Protein, GST, E.coli-100ug

QP5975-ec-100ug 100ug
EUR 489.6

Recombinant Human EIF4G1 Protein, GST, E.coli-10ug

QP5975-ec-10ug 10ug
EUR 240

Recombinant Human EIF4G1 Protein, GST, E.coli-1mg

QP5975-ec-1mg 1mg
EUR 1958.4

Recombinant Human EIF4G1 Protein, GST, E.coli-200ug

QP5975-ec-200ug 200ug
EUR 760.8

Recombinant Human EIF4G1 Protein, GST, E.coli-500ug

QP5975-ec-500ug 500ug
EUR 1272

Recombinant Human EIF4G1 Protein, GST, E.coli-50ug

QP5975-ec-50ug 50ug
EUR 315.6

Recombinant Human EIF4G1 Protein, His, Yeast-100ug

QP5975-ye-100ug 100ug
EUR 576

Recombinant Human EIF4G1 Protein, His, Yeast-10ug

QP5975-ye-10ug 10ug
EUR 283.2

Recombinant Human EIF4G1 Protein, His, Yeast-1mg

QP5975-ye-1mg 1mg
EUR 2262

Recombinant Human EIF4G1 Protein, His, Yeast-200ug

QP5975-ye-200ug 200ug
EUR 892.8

Recombinant Human EIF4G1 Protein, His, Yeast-500ug

QP5975-ye-500ug 500ug
EUR 1447.2

Recombinant Human EIF4G1 Protein, His, Yeast-50ug

QP5975-ye-50ug 50ug
EUR 358.8

Rabbit Anti-Rabbit EIF4G1 Polyclonal Antibody, Phospho-Ser1232

CPB-851RH 100 ul
EUR 670.8

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx133143
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx007344
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx000885
  • EUR 594.00
  • EUR 427.20
  • 100 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx112379
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

abx330400-100ul 100 ul
EUR 510

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx339291
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx326777
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

abx232724-100ug 100 ug
EUR 577.2

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody

20-abx211163
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)

1-CSB-EP007568HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in E.coli

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)

1-CSB-EP007568HUb9
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in E.coli

Rabbit Anti-Human EIF4G1 (aa 1230-1234) Polyclonal Antibody

CPB-1225RH 100 ul
EUR 552

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)

1-CSB-YP007568HU
  • EUR 516.00
  • EUR 280.80
  • EUR 1809.60
  • EUR 770.40
  • EUR 1210.80
  • EUR 349.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in Yeast

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0670905 3 x 1.0 ug
EUR 451.2

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4684105 3 x 1.0 ug
EUR 451.2

Human Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) ELISA Kit

abx387109-96tests 96 tests
EUR 1093.2

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0670906 1.0 ug DNA
EUR 200.4

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0670907 1.0 ug DNA
EUR 200.4

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0670908 1.0 ug DNA
EUR 200.4

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4684106 1.0 ug DNA
EUR 200.4

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4684107 1.0 ug DNA
EUR 200.4

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4684108 1.0 ug DNA
EUR 200.4

Eukaryotic Translation Initiation Factor 4 Gamma 1 Phospho-Ser1232 (EIF4G1 pS1232) Antibody

abx332891-100ul 100 ul
EUR 560.4

Eukaryotic Translation Initiation Factor 4 Gamma 1 Phospho-Ser1148 (EIF4G1 pS1148) Antibody

20-abx326530
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Similar quantities of biomass had been discovered (9.5 g and 9.four g L-1). Verbascoside manufacturing elevated parallel to cell development; maximal yield degree occurred within the mid-exponential part and lasted till the tip of the stationary part (i.e., from the 15th to the 25th day and from the ninth to the 21st day for the early and late phases, correspondingly).

The content material of VB was larger within the late-stage tradition (1.43 ± 0945 g L-1) than within the early-stage tradition (1.21 ± 0.0286 g L-1). Productivity values level out the potential use of Bcordata cell line within the biotechnological manufacturing of VB and for analysis targeted on the biochemistry of secondary metabolism.