Verbascoside production in long-term Buddleja cordata Kunth cell suspension cultures.

Previously, our group reported the institution of a white callus cell line of Buddleja cordata Kunth that may be a excessive producer of the secondary metabolite, verbascoside (VB, additionally named acteoside), below suspension tradition situations.

Here, we current experimental proof of the sustained capability of that mobile line to develop and produce excessive quantities of VB for five years of steady tradition. Cellular line profiles had been decided on the early (at first) and late phases (on the finish of 5 years of steady subculturing) by analyzing related parameters of tradition development, i.e., particular development charge [µ], doubling time [dt], and development index [GI], in addition to VB manufacturing.

Late-stage cultures exhibited a 61% sooner development charge than early-stage subcultures, and 25 and three% decrease doubling time and development index. The extents of development phases had been discovered to be totally different.

EIF4G1 Antibody
1-CSB-PA007999	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

EIF4G1 Antibody
1-CSB-PA780667	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against EIF4G1. Recognizes EIF4G1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

EIF4G1 Antibody
37544-100ul	SAB	100ul	EUR 252

EIF4G1 siRNA
20-abx915213	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

EIF4G1 siRNA
20-abx915214	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

EIF4G1 Antibody
ABD7614	Lifescience Market	100 ug	EUR 438

EIF4G1 Antibody
DF7614	Affbiotech	200ul	EUR 304
Description: EIF4G1 Antibody detects endogenous levels of total EIF4G1.

EIF4G1 antibody
70R-17060	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal EIF4G1 antibody

EIF4G1 antibody
70R-4875	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal EIF4G1 antibody raised against the middle region of EIF4G1

anti-EIF4G1
YF-PA11524	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to EIF4G1

anti-EIF4G1
YF-PA23636	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to EIF4G1

eIF4G1 Antibody
AF2641	Affbiotech	100ul	EUR 350

Anti-EIF4G1 antibody
STJ23520	St John's Laboratory	100 µl	EUR 393
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 antibody
STJ29690	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 antibody
STJ116294	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-EIF4G1 Antibody
A01351-2	BosterBio	100ug/vial	EUR 334

EIF4G1 Rabbit pAb
A0881-100ul	Abclonal	100 ul	EUR 384

EIF4G1 Rabbit pAb
A0881-200ul	Abclonal	200 ul	Ask for price

EIF4G1 Rabbit pAb
A0881-20ul	Abclonal	20 ul	Ask for price

EIF4G1 Rabbit pAb
A0881-50ul	Abclonal	50 ul	EUR 265

EIF4G1 (pS1148) Antibody
20-abx121476	Abbexa	EUR 314.00 EUR 467.00 EUR 203.00	100 ul 200 ul 30 ul

EIF4G1 (pS1232) Antibody
20-abx000330	Abbexa	EUR 495.00 EUR 704.00 EUR 356.00	100 ul 200 ul 50 ul

EIF4G1 Rabbit pAb
A7552-100ul	Abclonal	100 ul	EUR 308

EIF4G1 Rabbit pAb
A7552-200ul	Abclonal	200 ul	EUR 459

EIF4G1 Rabbit pAb
A7552-20ul	Abclonal	20 ul	EUR 183

EIF4G1 Rabbit pAb
A7552-50ul	Abclonal	50 ul	EUR 223

EIF4G1 Blocking Peptide
20-abx161839	Abbexa	EUR 272.00 EUR 411.00	1 mg 5 mg

anti- EIF4G1 antibody
FNab02724	FN Test	100µg	EUR 505.25
Description: Antibody raised against EIF4G1

EIF4G1 Conjugated Antibody
C37544	SAB	100ul	EUR 397

EIF4G1 Blocking Peptide
DF7614-BP	Affbiotech	1mg	EUR 195

anti-EIF4G1 (3A10)
LF-MA10096	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to EIF4G1

EIF4G1 Blocking Peptide
33R-6989	Fitzgerald	100 ug	EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EIF4G1 antibody, catalog no. 70R-4875

Anti-EIF4G1 antibody
PAab02724	Lifescience Market	100 ug	EUR 355

Anti-EIF4G1 (2A9)
YF-MA20178	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to EIF4G1

Phospho-EIF4G1 (Ser1232) Antibody
CSB-PA987086-	Cusabio		EUR 335
Description: A polyclonal antibody against Phospho-EIF4G1 (Ser1232). Recognizes Phospho-EIF4G1 (Ser1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Phospho-EIF4G1 (Ser1232) Antibody
CSB-PA987086-100ul	Cusabio	100ul	EUR 362
Description: A polyclonal antibody against Phospho-EIF4G1 (Ser1232). Recognizes Phospho-EIF4G1 (Ser1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Phospho-EIF4G1 (S1148) Antibody
1-CSB-PA007998	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against Phospho-EIF4G1 (S1148). Recognizes Phospho-EIF4G1 (S1148) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

EIF4G1 (Ab-1232) Antibody
CSB-PA061737-	Cusabio		EUR 335
Description: A polyclonal antibody against EIF4G1 (Ab-1232). Recognizes EIF4G1 (Ab-1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

EIF4G1 (Ab-1232) Antibody
CSB-PA061737-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against EIF4G1 (Ab-1232). Recognizes EIF4G1 (Ab-1232) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Mouse EIF4G1 shRNA Plasmid
20-abx980560	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human EIF4G1 shRNA Plasmid
20-abx951370	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

EIF4G1 (pS1148) Blocking Peptide
20-abx162409	Abbexa	EUR 314.00 EUR 509.00	1 mg 5 mg

Mouse Eif4g1 ELISA KIT
ELI-42147m	Lifescience Market	96 Tests	EUR 865

EIF4G1 ELISA KIT|Human
EF009355	Lifescience Market	96 Tests	EUR 689

Human EIF4G1 ELISA KIT
ELI-13400h	Lifescience Market	96 Tests	EUR 824

Anti-Phospho-EIF4G1-(S1108) antibody
STJ117894	St John's Laboratory	100 µl	EUR 393
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Anti-Phospho-EIF4G1-(S1232) antibody
STJ22122	St John's Laboratory	100 µl	EUR 393
Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Phospho-EIF4G1-S1232 Rabbit pAb
AP0279-100ul	Abclonal	100 ul	EUR 384

Phospho-EIF4G1-S1232 Rabbit pAb
AP0279-200ul	Abclonal	200 ul	EUR 554

Phospho-EIF4G1-S1232 Rabbit pAb
AP0279-20ul	Abclonal	20 ul	Ask for price

Phospho-EIF4G1-S1232 Rabbit pAb
AP0279-50ul	Abclonal	50 ul	EUR 265

Phospho-EIF4G1-S1108 Rabbit pAb
AP0796-100ul	Abclonal	100 ul	EUR 384

Phospho-EIF4G1-S1108 Rabbit pAb
AP0796-200ul	Abclonal	200 ul	EUR 554

Phospho-EIF4G1-S1108 Rabbit pAb
AP0796-20ul	Abclonal	20 ul	EUR 183

Phospho-EIF4G1-S1108 Rabbit pAb
AP0796-50ul	Abclonal	50 ul	EUR 265

Eif4g1 ORF Vector (Mouse) (pORF)
ORF043791	ABM	1.0 ug DNA	EUR 1572

Eif4g1 ORF Vector (Mouse) (pORF)
ORF043792	ABM	1.0 ug DNA	EUR 1572

EIF4G1 ORF Vector (Human) (pORF)
ORF018839	ABM	1.0 ug DNA	EUR 659

EIF4G1 sgRNA CRISPR Lentivector set (Human)
K0670901	ABM	3 x 1.0 ug	EUR 339

Eif4g1 sgRNA CRISPR Lentivector set (Mouse)
K4684101	ABM	3 x 1.0 ug	EUR 339

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 1)
K0670902	ABM	1.0 ug DNA	EUR 154

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 2)
K0670903	ABM	1.0 ug DNA	EUR 154

EIF4G1 sgRNA CRISPR Lentivector (Human) (Target 3)
K0670904	ABM	1.0 ug DNA	EUR 154

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4684102	ABM	1.0 ug DNA	EUR 154

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4684103	ABM	1.0 ug DNA	EUR 154

Eif4g1 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4684104	ABM	1.0 ug DNA	EUR 154

Eif4g1 3'UTR Luciferase Stable Cell Line
TU105738	ABM	1.0 ml	Ask for price

EIF4G1 Protein Vector (Human) (pPB-C-His)
PV075353	ABM	500 ng	EUR 2496

EIF4G1 Protein Vector (Human) (pPB-N-His)
PV075354	ABM	500 ng	EUR 2496

EIF4G1 Protein Vector (Human) (pPM-C-HA)
PV075355	ABM	500 ng	EUR 2496

EIF4G1 Protein Vector (Human) (pPM-C-His)
PV075356	ABM	500 ng	EUR 2496

Recombinant Human EIF4G1 Protein, GST, E.coli-100ug
QP5975-ec-100ug	EnQuireBio	100ug	EUR 408

Recombinant Human EIF4G1 Protein, GST, E.coli-10ug
QP5975-ec-10ug	EnQuireBio	10ug	EUR 200

Recombinant Human EIF4G1 Protein, GST, E.coli-1mg
QP5975-ec-1mg	EnQuireBio	1mg	EUR 1632

Recombinant Human EIF4G1 Protein, GST, E.coli-200ug
QP5975-ec-200ug	EnQuireBio	200ug	EUR 634

Recombinant Human EIF4G1 Protein, GST, E.coli-500ug
QP5975-ec-500ug	EnQuireBio	500ug	EUR 1060

Recombinant Human EIF4G1 Protein, GST, E.coli-50ug
QP5975-ec-50ug	EnQuireBio	50ug	EUR 263

Recombinant Human EIF4G1 Protein, His, Yeast-100ug
QP5975-ye-100ug	EnQuireBio	100ug	EUR 480

Recombinant Human EIF4G1 Protein, His, Yeast-10ug
QP5975-ye-10ug	EnQuireBio	10ug	EUR 236

Recombinant Human EIF4G1 Protein, His, Yeast-1mg
QP5975-ye-1mg	EnQuireBio	1mg	EUR 1885

Recombinant Human EIF4G1 Protein, His, Yeast-200ug
QP5975-ye-200ug	EnQuireBio	200ug	EUR 744

Recombinant Human EIF4G1 Protein, His, Yeast-500ug
QP5975-ye-500ug	EnQuireBio	500ug	EUR 1206

Recombinant Human EIF4G1 Protein, His, Yeast-50ug
QP5975-ye-50ug	EnQuireBio	50ug	EUR 299

EIF4G1 Protein Vector (Mouse) (pPB-C-His)
PV175162	ABM	500 ng	EUR 2690

EIF4G1 Protein Vector (Mouse) (pPB-N-His)
PV175163	ABM	500 ng	EUR 2690

EIF4G1 Protein Vector (Mouse) (pPM-C-HA)
PV175164	ABM	500 ng	EUR 2690

EIF4G1 Protein Vector (Mouse) (pPM-C-His)
PV175165	ABM	500 ng	EUR 2690

EIF4G1 Protein Vector (Mouse) (pPB-C-His)
PV175166	ABM	500 ng	EUR 2679

EIF4G1 Protein Vector (Mouse) (pPB-N-His)
PV175167	ABM	500 ng	EUR 2679

EIF4G1 Protein Vector (Mouse) (pPM-C-HA)
PV175168	ABM	500 ng	EUR 2679

EIF4G1 Protein Vector (Mouse) (pPM-C-His)
PV175169	ABM	500 ng	EUR 2679

Eif4g1 3'UTR GFP Stable Cell Line
TU253905	ABM	1.0 ml	Ask for price

Eif4g1 3'UTR Luciferase Stable Cell Line
TU203905	ABM	1.0 ml	Ask for price

EIF4G1 3'UTR GFP Stable Cell Line
TU056792	ABM	1.0 ml	EUR 2333

EIF4G1 3'UTR Luciferase Stable Cell Line
TU006792	ABM	1.0 ml	EUR 2333

Eif4g1 3'UTR GFP Stable Cell Line
TU155738	ABM	1.0 ml	Ask for price

Rabbit Anti-Rabbit EIF4G1 Polyclonal Antibody, Phospho-Ser1232
CPB-851RH	Creative Diagnostics	100 ul	EUR 559

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)
1-CSB-YP007568HU	Cusabio	EUR 430.00 EUR 234.00 EUR 1508.00 EUR 642.00 EUR 1009.00 EUR 291.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in Yeast

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)
1-CSB-EP007568HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in E.coli

Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)
1-CSB-EP007568HUb9	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 4 gamma 1(EIF4G1),partial expressed in E.coli

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx112379	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Rabbit Anti-Human EIF4G1 (aa 1230-1234) Polyclonal Antibody
CPB-1225RH	Creative Diagnostics	100 ul	EUR 460

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx339291	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx133143	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx000885	Abbexa	EUR 495.00 EUR 356.00	100 ul 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx007344	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx211163	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
abx232724-100ug	Abbexa	100 ug	EUR 481

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
abx330400-100ul	Abbexa	100 ul	EUR 425

Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) Antibody
20-abx326777	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K0670905	ABM	3 x 1.0 ug	EUR 376

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4684105	ABM	3 x 1.0 ug	EUR 376

Human Eukaryotic Translation Initiation Factor 4 Gamma 1 (EIF4G1) ELISA Kit
abx387109-96tests	Abbexa	96 tests	EUR 911

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K0670906	ABM	1.0 ug DNA	EUR 167

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K0670907	ABM	1.0 ug DNA	EUR 167

EIF4G1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K0670908	ABM	1.0 ug DNA	EUR 167

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4684106	ABM	1.0 ug DNA	EUR 167

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4684107	ABM	1.0 ug DNA	EUR 167

Eif4g1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4684108	ABM	1.0 ug DNA	EUR 167

Eukaryotic Translation Initiation Factor 4 Gamma 1 Phospho-Ser1232 (EIF4G1 pS1232) Antibody
abx332891-100ul	Abbexa	100 ul	EUR 467

Eukaryotic Translation Initiation Factor 4 Gamma 1 Phospho-Ser1148 (EIF4G1 pS1148) Antibody
20-abx326530	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Similar quantities of biomass had been discovered (9.5 g and 9.four g L^-1). Verbascoside manufacturing elevated parallel to cell development; maximal yield degree occurred within the mid-exponential part and lasted till the tip of the stationary part (i.e., from the 15th to the 25th day and from the ninth to the 21st day for the early and late phases, correspondingly).

The content material of VB was larger within the late-stage tradition (1.43 ± 0945 g L^-1) than within the early-stage tradition (1.21 ± 0.0286 g L^-1). Productivity values level out the potential use of B. cordata cell line within the biotechnological manufacturing of VB and for analysis targeted on the biochemistry of secondary metabolism.