Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes.

Bdellovibrio and like organisms (BALOs) are obligate predatory micro organism that selectively prey on a broad vary of Gram-negative micro organism, together with multidrug-resistant human pathogens. Due to their distinctive way of life, they’ve been lengthy acknowledged as a possible therapeutic and biocontrol agent.

Research on BALOs has quickly grown over the latest decade, leading to many publications regarding molecular particulars of bacterial predation in addition to purposes thereof in medication and biotechnology.

This evaluation summarizes the present information on biotechnological potential of obligate predatory micro organism and their secreted enzymes.

Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes.
Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes.

The Cellular Response to Lanthanum Is Substrate Specific and Reveals a Novel Route for Glycerol Metabolism in Pseudomonas putida KT2440.

Ever because the discovery of the primary uncommon earth component (REE)-dependent enzyme, the physiological function of lanthanides has grow to be an rising discipline of analysis because of the environmental implications and biotechnological alternatives. In Pseudomonas putida KT2440, the 2 pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs) PedE and PedH are inversely regulated in response to REE availability. This transcriptional swap is orchestrated by a fancy regulatory community that features the PedR2/PedS2 two-component system and is essential for environment friendly progress on a number of alcoholic volatiles.

To examine whether or not mobile responses past the REE swap exist, the differential proteomic responses that happen throughout progress on varied mannequin carbon sources had been analyzed. Apart from the Ca2+-dependent enzyme PedE, the differential abundances of most recognized proteins had been conditional. During progress on glycerol-and concomitant with the proteomic changes-lanthanum (La3+) availability affected totally different progress parameters, together with the onset of logarithmic progress and ultimate optical densities. Studies with mutant strains revealed a novel metabolic route for glycerol utilization, initiated by PedE and/or PedH exercise.

EIF5A

pro-674 5µg
EUR 60
Description: Recombinant Human Eukaryotic Translation Initiation Factor 5A

eIF5A

E8ET1610-49 100ul
EUR 275
Description: Available in various conjugation types.

EIF5A

E8EM1706-1 100ul
EUR 275
Description: Available in various conjugation types.

EIF5A

CSB-CL007573HU 10 μg plasmid + 200μl Glycerol Ask for price

EIF5A siRNA

20-abx915224
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF5A siRNA

20-abx915225
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF5A siRNA

20-abx901691
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF5A antibody

70R-17064 50 ul
EUR 522
Description: Rabbit polyclonal EIF5A antibody

EIF5A antibody

70R-33144 100 ug
EUR 522
Description: Rabbit polyclonal EIF5A antibody

EIF5A antibody

70R-49699 100 ul
EUR 292.8
Description: Purified Polyclonal EIF5A antibody

EIF5A Antibody

32552-100ul 100ul
EUR 302.4

eIF5A Antibody

48963-100ul 100ul
EUR 399.6

eIF5A Antibody

48963-50ul 50ul
EUR 286.8

EIF5A Antibody

ABD6754 100 ug
EUR 525.6

EIF5A Antibody

1-CSB-PA007573GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against EIF5A. Recognizes EIF5A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

EIF5A Antibody

R35713-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Eukaryotic translation initiation factor 5A

EIF5A Antibody

RQ6620 100ug
EUR 356.15
Description: Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs. The eIF5A (eIF5A1) and eIF5A2 genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation. Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding AMD1 and eIF5A genes often occur together in human lymphomas.

EIF5A Antibody

RQ6981 100 ug
EUR 356.15
Description: Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs. The eIF5A (eIF5A1) and eIF5A2 genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation. Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding AMD1 and eIF5A genes often occur together in human lymphomas.

EIF5A Antibody

E10-30713 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E10-30713T 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E10-30744 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E10-30744T 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E313344 200ul
EUR 275
Description: Available in various conjugation types.

EIF5A antibody

E39-02728 100ug/100ul
EUR 225
Description: Available in various conjugation types.

EIF5A antibody

E39-10466 100ug/100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E38PA1380 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Antibody

E560007-A-SE 100ug
EUR 295
Description: Available in various conjugation types.

EIF5A Antibody

E92016 100μg
EUR 255
Description: Available in various conjugation types.

eIF5A Antibody

48963 100ul
EUR 499

EIF5A Antibody

32552 100ul
EUR 439

pSV40- Eif5a- m

PVT11661 2 ug
EUR 327.6

eIF5A/eIF5A2 Polyclonal Antibody

E28PT6284 100μl
EUR 255
Description: Available in various conjugation types.

EIF5A Mouse mAb

E2221176 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Mouse mAb

E2221177 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Mouse mAb

E2221230 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Mouse mAb

E2221231 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Rabbit pAb

A2016-100ul 100 ul
EUR 369.6

EIF5A Rabbit pAb

A2016-200ul 200 ul
EUR 550.8

EIF5A Rabbit pAb

A2016-20ul 20 ul
EUR 219.6

EIF5A Rabbit pAb

A2016-50ul 50 ul
EUR 267.6

eIF5A Rabbit mAb

A4414-100ul 100 ul
EUR 492

eIF5A Rabbit mAb

A4414-200ul 200 ul
EUR 685.2

eIF5A Rabbit mAb

A4414-20ul 20 ul
EUR 265.2

eIF5A Rabbit mAb

A4414-50ul 50 ul
EUR 344.4

eIF5A Rabbit mAb

A4414 500μL
EUR 1557.4
Description: Recombinant protein of mouse CD23

eIF5A Rabbit pAb

E2381193 100ul
EUR 225
Description: Available in various conjugation types.

EIF5A Rabbit pAb

E2502016 100ul
EUR 225
Description: Available in various conjugation types.

eIF5A Rabbit mAb

E2R23339 100ul
EUR 255
Description: Available in various conjugation types.

eIF5A Rabbit mAb

58920 100ul
EUR 339

anti- EIF5A antibody

FNab02728 100µg
EUR 658.5
Description: Antibody raised against EIF5A

EIF5A cloning plasmid

CSB-CL007573HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the EIF5A gene.

EIF5A Blocking Peptide

20-abx062574
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

EIF5A Blocking Peptide

DF6754-BP 1mg
EUR 234

eIF5A Conjugated Antibody

C48963 100ul
EUR 476.4

EIF5A Conjugated Antibody

C32552 100ul
EUR 476.4

Eif5a (GFP-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a)

MG201137 10 µg Ask for price

EIF5A ELISA KIT|Human

EF009359 96 Tests
EUR 826.8

Human EIF5A ELISA KIT

ELI-20280h 96 Tests
EUR 988.8

Mouse Eif5a ELISA KIT

ELI-20519m 96 Tests
EUR 1038

Bovine EIF5A ELISA KIT

ELI-20279b 96 Tests
EUR 1113.6

Rat EIF5A shRNA Plasmid

20-abx988241
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human EIF5A shRNA Plasmid

20-abx951373
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse EIF5A shRNA Plasmid

20-abx983157
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

EIF5A Recombinant Protein (Rat)

RP199424 100 ug Ask for price

OPPA01885-5UG - EIF5A Protein

OPPA01885-5UG 5ug
EUR 70

OAPA00346-5UG - EIF5A Antibody

OAPA00346-5UG 5ug
EUR 69

Eif5a (untagged ORF) - Rat eukaryotic translation initiation factor 5A (Eif5a), (10 ug)

RN215547 10 µg Ask for price

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

ABP50097-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Acetyl eIF5A/eIF5A2 K47) from Human, Mouse, Rat. This Acetyl eIF5A/eIF5A2 K47) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human eIF5A/eIF5A2 around the acetylation site of K47

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

ABP50097-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Acetyl eIF5A/eIF5A2 K47) from Human, Mouse, Rat. This Acetyl eIF5A/eIF5A2 K47) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human eIF5A/eIF5A2 around the acetylation site of K47

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

ABP50097-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Acetyl eIF5A/eIF5A2 K47) from Human, Mouse, Rat. This Acetyl eIF5A/eIF5A2 K47) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human eIF5A/eIF5A2 around the acetylation site of K47

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

ES1096-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Acetyl eIF5A/eIF5A2 (K47) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

ES1096-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Acetyl eIF5A/eIF5A2 (K47) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

E20-40021 100ug
EUR 225
Description: Available in various conjugation types.

OAAN00669-50UL - EIF5A Antibody

OAAN00669-50UL 50ul
EUR 149

EIF5A Recombinant Protein (Human)

RP010498 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131399 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131402 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131405 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131408 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131411 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131414 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131417 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131420 100 ug Ask for price

EIF5A Recombinant Protein (Mouse)

RP131423 100 ug Ask for price

OAGA03681-100UL - EIF5A Antibody

OAGA03681-100UL 100ul
EUR 369

OAEB01157-100UG - EIF5A Antibody

OAEB01157-100UG 100ug
EUR 349

OAAJ07228-100UL - EIF5A Antibody

OAAJ07228-100UL 100uL
EUR 319

OAAN00669-100UL - EIF5A Antibody

OAAN00669-100UL 100ul
EUR 249

OAAN00669-200UL - EIF5A Antibody

OAAN00669-200UL 200ul
EUR 389

ARP88522_P050 - EIF5A Antibody (ARP88522_P050)

ARP88522_P050 100ul
EUR 389

Eif5a ORF Vector (Rat) (pORF)

ORF066476 1.0 ug DNA
EUR 607.2

eIF5A recombinant monoclonal antibody

A5650 100ul X 3
EUR 714
Description: A recombinant monoclonal antibody from rabbit against human eIF5A for WB, IHC,ELISA

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant C

SC324740 10 µg Ask for price

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant D

SC324741 10 µg Ask for price

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant A

SC325573 10 µg Ask for price

EIF5A (untagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B

SC118910 10 µg Ask for price

EIF5A ORF Vector (Human) (pORF)

ORF003500 1.0 ug DNA
EUR 114

Eif5a ORF Vector (Mouse) (pORF)

ORF043801 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043802 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043803 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043804 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043805 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043806 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043807 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043808 1.0 ug DNA
EUR 607.2

Eif5a ORF Vector (Mouse) (pORF)

ORF043809 1.0 ug DNA
EUR 607.2

[One Step] EIF5A Antibody Kit

RK05645 50 ul
EUR 288

EIF5A ELISA Kit (Human) (OKEH08524)

OKEH08524 96 Wells
EUR 1075.2
Description: Description of target: ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.163ng/mL

Human EIF5A Protein Lysate 20ug

IHUEIF5APLLY20UG each
EUR 213
Description: Human EIF5A Protein Lysate 20ug

EIF5A (GFP-tagged) - Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant A

RG226528 10 µg Ask for price

EIF5A (GFP-tagged) - Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant C

RG226600 10 µg Ask for price

EIF5A (GFP-tagged) - Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant D

RG226664 10 µg Ask for price

EIF5A (GFP-tagged) - Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B

RG200770 10 µg Ask for price

Eif5a (untagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 5, (10ug)

MC202889 10 µg Ask for price

Eif5a (untagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 9, (10ug)

MC207032 10 µg Ask for price

Eif5a (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 5A (Eif5a), (10 ug)

RR215547 10 µg Ask for price

Anti-eIF5A/eIF5A2 (Acetyl K47) antibody

STJ90136 200 µl
EUR 236.4
Description: Rabbit polyclonal to Acetyl-eIF5A/eIF5A2 (K47).

Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody

A05688 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Acetyl-eIF5A/eIF5A2 (K47) Antibody (EIF5A2) detection.tested for WB in Human, Mouse, Rat.

OCOA14350-20UG - EIF5A Protein Lysate

OCOA14350-20UG 20ug
EUR 169

OCOA19192-20UG - EIF5A Protein Lysate

OCOA19192-20UG 20ug
EUR 169

OCOA19193-20UG - EIF5A Protein Lysate

OCOA19193-20UG 20ug
EUR 169

Monoclonal EIF5A Antibody, Clone: 4E10G8

APG03268G 0.1ml
EUR 633.6
Description: A Monoclonal antibody against Human EIF5A. The antibodies are raised in Mouse and are from clone 4E10G8. This antibody is applicable in WB and IHC, FC, ICC, E

Anti-Acetylated eIF5A (Lys47) Antibody

P01727 100ul
EUR 476.4
Description: Rabbit Polyclonal Acetylated eIF5A (Lys47) Antibody. Validated in WB and tested in Human, Mouse, Rat.

Rabbit Polyclonal Antibody against EIF5A

TA300510 100 µl Ask for price

EIF5A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B

RC200770 10 µg Ask for price

EIF5A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant A

RC226528 10 µg Ask for price

EIF5A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant C

RC226600 10 µg Ask for price

EIF5A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A (EIF5A), transcript variant D

RC226664 10 µg Ask for price

Eif5a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 1

MR226057 10 µg Ask for price

Eif5a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5A (Eif5a), transcript variant 2

MR226059 10 µg Ask for price

Upon oxidation to glycerate by way of glyceraldehyde, phosphorylation by the glycerate kinase GarK almost definitely yields glycerate-2-phosphate, which is finally channeled into the central metabolism of the cell. This new route capabilities in parallel with the primary degradation pathway encoded by the glpFKRD operon and offers a progress benefit to the cells by permitting an earlier onset of progress with glycerol as the only real supply of carbon and power.IMPORTANCE The organic function of REEs has lengthy been underestimated, and analysis has primarily targeted on methanotrophic and methylotrophic micro organism.

We have lately demonstrated that P. putida, a plant growth-promoting bacterium that thrives within the rhizosphere of varied meals crops, possesses a REE-dependent alcohol dehydrogenase (PedH), however information about REE-specific results on physiological traits in nonmethylotrophic micro organism remains to be scarce. This examine demonstrates that the mobile response of P. putida to lanthanum (La3+) is generally substrate particular and that La3+ availability extremely impacts the expansion of cells on glycerol.

Further, a novel route for glycerol metabolism is recognized, which is initiated by PedE and/or PedH exercise and offers a progress benefit to this biotechnologically related organism by permitting a sooner onset of progress. Overall, these findings reveal that lanthanides can have an effect on physiological traits in nonmethylotrophic micro organism and may affect their competitiveness in varied environmental niches.

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